Offre de stage : Organoïde de tendon construit à partir de matériaux électrospinnés / Tendon organoid on electrospun materials

Sujet : Organoïde de tendon construit à partir de matériaux électrospinnés / Tendon
organoid on electrospun materials

Laboratoire/équipe : UMR CNRS 7338 Bio­mé­canique et Bio­ingénierie – Equipes CBB / C2MUST

Mots clés : Elec­tro­spin­ning, ingénierie tis­su­laire, matrice extra­cel­lu­laire

Back­ground: Up to now, ten­don engi­neer­ing has been devel­oped in the frame­work of regen­er­a­tive med­i­cine, but not applied to pre­clin­i­cal drug screen­ing.

Project aim: Gen­er­ate a ten­don organoid by the cul­ture of prog­en­i­tor cells on elec­tro­spun matrix made of Nylon or oth­er mate­ri­als under sta­t­ic and dynam­ic con­di­tions. Char­ac­ter­ize the neo-syn­the­sized extra­cel­lu­lar matrix (ECM).

Project descrip­tion: The elec­tro­spun scaf­fold will be pre­pared fol­low­ing pro­to­cols pre­vi­ous­ly val­i­dat­ed in the lab. Ten­don cells or prog­en­i­tors will be seed­ed on Nylon elec­tro­spun scaf­folds and cul­tured under sta­t­ic or dynam­ic (stretch­ing con­di­tions). Ten­don organoids will be char­ac­ter­ized from both mechan­i­cal and bio­log­i­cal aspects. The com­po­si­tion and spa­tial orga­ni­za­tion of the
neosyn­the­sized extra­cel­lu­lar matrix will be assessed with immuno­his­to­chem­istry. The local Young mod­u­lus (E) of each scaf­fold will be mea­sured with a Chiaro nanoin­den­ter sys­tem (Optics 11) mount­ed on an opti­cal micro­scope. AFM mea­sure­ments will also be per­formed to deter­mine elas­tic and vis­coelas­tic prop­er­ties of bio­hy­brid mate­ri­als at the fiber scale.
Depend­ing on the project’s progress, we also plan to alter col­la­gen and/or the whole extra­cel­lu­lar matrix by gly­ca­tion, to mim­ic an altered ten­don due to dia­betis or age­ing.

Project impact: Once the organoid will be pro­duced and char­ac­ter­ized, spe­cif­ic
drugs will be applied to eval­u­ate alter­ations of the tis­sue. This should lead to a
new in vit­ro eval­u­a­tion tool as an alter­na­tive method to ani­mal trials.